Cation Content in Poliovirus-infected HeLa Cells
نویسندگان
چکیده
منابع مشابه
Alterations in initiation factor activity from poliovirus-infected HeLa cells.
Ribosomal salt washes were prepared from uninfected and poliovirus-infected HeLa cells and separated by fractional (NH&SO* precipitation into an A (0 to 40% saturation) and a B (40 to 70%) fraction. Initiation factor activity in the washes and A and B fractions was assayed in endogenous mRNA translation systems derived from HeLa cells. The wash from uninfected cells stimulated the translation o...
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The Na+ content of poliovirus-infected HeLa S3 cells increased during the late phase of virus replication, after virus inhibition of host cell protein synthesis and in coincidence with late viral functions. Guanidine hydrochloride blocked the rise in Na+ content, whereas the antiguanidine agent choline fully reversed the guanidine block. Expression of one or more late viral functions was essent...
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Brefeldin A (BFA) is a macrolide antibiotic that blocks membrane traffic through the vesicular system and affects the glycosylation of viral glycoproteins. Treatment of HeLa cells infected with Semliki Forest virus (SFV) with BFA enhances the synthesis of late viral proteins. Proteolytic cleavage of p107 is partially blocked and viral glycoproteins accumulate in BFA-treated cells. This enhanced...
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In poliovirus-infected HeLa cells, the mechanism of protein synthesis initiation factor recognition of m7G cap groups on mRNA is impaired. Translation of capped host cell mRNAs is inhibited, whereas translation of uncapped poliovirus mRNA proceeds exclusively. The site of this defect has been localized to the cap-binding protein complex (CBPC). To elucidate the specific structural and functiona...
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A portion of poliovirus double-stranded RNA (25 to 50%) isolated from infected HeLa cells contains hairpin loops at one end of the duplex structure. These structures rapidly reformed double-stranded molecules after denaturation and appeared as molecules of up to two times genome length upon electrophoresis in denaturing agarose gels. A second form of poliovirus double-stranded RNA was readily d...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1987
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-68-2-335